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Atropine is primarily used as an antidote in situations involving exposure to certain nerve agents. Nerve agents disrupt the normal functioning of the nervous system by inhibiting the enzyme acetylcholinesterase, leading to a buildup of the neurotransmitter acetylcholine, which can result in a life-threatening overstimulation of muscles and glands. Atropine works by blocking the action of acetylcholine at muscarinic receptors, helping to alleviate the symptoms caused by nerve agent exposure, such as excessive salivation, bronchial secretions, and muscle spasms.

In specific medical situations, atropine is also administered during resuscitation efforts to help restore normal heart rhythm, but its role in counteracting the toxic effects of nerve agents is its most recognized application.